摘要 |
5-methoxy-7-hydroxyflavan compound (I) was isolated and purified from Dragon's Blood resin. The purification process involves 50% ethanol soxhletion overnight and then fraction through silica column with a series of solvent elution. The purified compound (I) was crystallized in benzene: hexane (1:1). The pure compound was identified by instruments including IR, NMR, FAB-MS, and GC-MS and acetylation reaction to confirm the structure. This compound was found to be a strong antifungal chemical with broad spectra against Fusarium oxysporium fsp. niveum, Phytophora capsici, Phoma asparagi, Pythium spinosum, Pythium aphanidermatum, Phthium sylvaticum, Sclerotium rolfsii, and Sclerotinia sclerotiorum . For whole plant in vivo testes, it was found that compound (I) significantly protected mung bean and cabbage from being infested and damaged by Sclerotium rolfsii and Pythium aphanidermatum respectively. Toxicology experiments with compound (I), it shown no toxcity or effect to animals by peroral 300 mg/kg or intraperitoneal 100 mg/kg (by Brion Research Institute of Taiwan). It is a definite conclusion that compound is a safe fungicide in agriculture. |