IMMUNOSELECTION DEVICE AND METHOD

摘要

Devices and methods are provided for selectively isolating or separating target particles from a mixture of target particles and non-target particles. The devices are suitable for the separation of target particles which are indirectly bound to a first member, from a mixture of target particles and non-target particles, comprising: a column having a proximal end with an inlet port through which fluid may exit the column; and a bed of low nonspecific binding porous material within the column, the porous material having a second member capable of binding to the first member with an affinity constant of greater than about 10<8>M<-1> immobilized on the surface thereof, wherein the pores of the porous material are of a size sufficient to allow the second member to enter into the pores and to allow entry of molecules greater than about 35 kD, but not so large as to allow collapse of the bed, and wherein the interstitial spaces of the bed are of a size sufficient to allow the particles to flow through the bed.