| 摘要 |
PURPOSE:To remove a substance derived from Escherichia coli from an aqueous solution of genetic recombinant proteins, etc., by bringing an aqueous solution of the substance derived from the Escherichia coli into contact with a strong basic anion exchange resin and adsorbing the substance derived from the Escherichia coli on the aforementioned resin. CONSTITUTION:An aqueous solution containing both microbial cell proteins derived from Escherichia coli and pyrogens is brought into contact with a strong basic anion exchange resin to adsorb a substance derived from the Escherichia coli on the aforementioned resin and isolate the substance derived from the Escherichia coli. The aqueous solution containing the substance derived from the Escherichia coli is normally an aqueous solution containing proteins produced by genetic recombination using the Escherichia coli as a host. In this case, a supernatant or partially purified aqueous solution after crushing the microbial cells is cited as the aqueous solution. A strong basic anion exchange resin mainly having quaternary amines as exchange groups is preferably used as the strong basic anion exchange resin. |
