METHOD FOR SEPARATING AND CONCENTRATING PHOSPHOPEPTIDE

摘要

PURPOSE:To efficiently separate and concentrate a phosphopeptide by reacting casein with a protease, adsorbing the resultant protein hydrolyzate on a crosslinked and formed product of chitosan and then desorbing the hydrolyzate from the aforementioned formed product. CONSTITUTION:Chitosan is formed into a cellular granular substance, membrane, etc., and subjected to crosslinking treatment with a crosslinking agent such as a diisocyanate to provide a crosslinked and formed product of chitosan with acid tolerance imparted thereto. A protease is then allowed to react with a raw material composed of casein and/or based on the chasein to produce a protein hydrolyzate. The produced protein hydrolyzate is subsequently adsorbed on the above-mentioned crosslinked and formed product of the chitosan and then desorbed to separate and concentrate a phosphopeptide. The pH is adsorbing the protein hydrolyzate on the crosslinked and formed product of the chitosan is preferably within the range of 1.5-5.0.