ENZYMATIC SYNTHESIS OF SOLUBLE PHOSPHATIDES FROM PHOSPHOLIPIDS
摘要
A method of preparing a soluble monovalent salt of a phosphatidyl ester which comprises reacting a phospholipid with a primary alcohol in the presence of an enzyme catalyst in a divalent cationic buffered solution and a water immiscible non-ether solvent that does not inactivate the enzyme, to form a divalent cationic salt of the phosphatidyl ester, and suspending the product in the presence of a stoichiometric amount of a monovalent cationic salt whose anion forms an insoluble salt with the divalent cation. The use of Centrifugal Partition Chromatography facilitates the enzyme reaction. The monovalent salt is preferably an ammonium/sodium mixed salt.
申请公布号
WO9116444(A1)
申请公布日期
1991.10.31
申请号
WO1991US02608
申请日期
1991.04.16
申请人
THE LIPOSOME COMPANY, INC.
发明人
TREMBLAY, PAUL, A.;MARZIANI, FRANK;TINO, JOHN, A., F.;PILKIEWICZ, FRANK, G.