发明名称 |
IN VITRO DNA SYNTHESIS REACTIONS USING PHI 29 DNA POLYMERASE AND A DNA FRAGMENT ENCODING SAID POLYMERASE |
摘要 |
An improved method for determining the nucleotide base sequence of a DNA molecule. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each of the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA polymerase which is a PHI 29-type DNA polymerase. |
申请公布号 |
WO9116446(A1) |
申请公布日期 |
1991.10.31 |
申请号 |
WO1990US01631 |
申请日期 |
1990.04.13 |
申请人 |
CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS;UNITED STATES BIOCHEMICHAL CORPORATION |
发明人 |
BLANCO, LUIS;BERNAD, ANTONIO;SALAS, MARGARITA |
分类号 |
C07H15/12;C07H17/00;C12N9/12;C12N15/09;C12N15/10;C12N15/54;C12P19/34;C12Q1/68;C12Q1/70;G01N33/566 |
主分类号 |
C07H15/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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