| 摘要 |
<p>A method of expressing a gene in an intact, unfused form comprises amplification of the gene using Polymerase Chain Reaction (PCR) starting from the second codon of the particular gene and ligating the resulting product to the ATG codon which is provided by an Ncol site of an expression vector. Using this strategy high level expression fo Plasmodium falciparum hypoxanthine guanine phosphoribosyl transferase (Pf HGPRT) gene, in E.coli, has been achieved. Vectors for use in the method are also disclosed.</p> |
