ENZYMATIC SYNTHESIS OF SOLUBLE PHOSPHATIDES FROM¹PHOSPHOLIPIDS

摘要

The present invention relates to a process for forming a soluble monovalent salt of a phosphatidyl ester which comprises : a) reacting a phospholipid with a primary alcohol in the presence of ; i) an enzyme catalyst phospholipase D ; ii) a divalent cationic buffer solution and iii) a water immiscible non-ether solvent so as to form the corresponding phosphatide of the divalent cationic salt; and b) converting the divalent cationic salt to an organic soluble monovalent salt by suspending with a stoichiometric amount of a monovalent salt having a monovalent cation and whose anion forms an insoluble precipitate with the divalent cation.