| 摘要 |
NEW MATERIAL:A protein having peptide chains of formulas I and II and a salt thereof. USE:A sweetener. The protein has highly strong sweetness compared with sucrose. PREPARATION:Prescribed amino acids are successively bonded to a carrier resin in the presence of a condensing agent such as dicylohexyl carbodiimide according to a solid synthetic method and the bond of the resin to the C terminal of the produced peptide is cleaved. Peptide chains of formulas I and II are synthesized by the above-mentioned method, respectively. The synthesized peptide chain of formula I is purified, dissolved in a 0.1% acetic acid solution and subsequently mixed with the synthesized and purified peptide of formula II to form a complex, which is separated and purified by a reverse phase liquid chromatography, etc. |
