Cholesterol oxidase.

摘要

A novel cholesterol oxidase is disclosed having a high substrate affinity, high activity and operable over a wide pH range, including acid value. The enzyme is produced in low yield by microorganisms of the species Brevibacterium sterolicum. Improved yields are obtained by transforming microorganisms of the species E.coli with the DNA from Brevibacterium sterolicum and which encodes the novel enzyme.