| 摘要 |
<p>A method for identifying an inhibitor of the transcription activation activity of a papilloma E6 peptide. The method includes providing (a) a hybrid peptide including a papillomavirus E6 peptide having the transcription activation activity of the E6 peptide, covalently bonded with a heterologous nucleic acid binding domain having specific nucleic acid binding activity; (b) nucleic acid encoding a detectable protein in transcription relationship with nucleic acid to which the heterologous nucleic acid binding domain binds; and (c) a potential inhibitor. These three components are contacted together to cause transcription of the nucleic acid to produce a predetermined level of the detectable protein when the potential inhibitor is inactive to inhibit the transcription activation activity of that peptide; and a lower level of the detectable protein when the potential inhibitor is active to inhibit the transcription activation activity.</p> |
