| 摘要 |
A spectrofluorometry apparatus utilizes dual wavelengths. The dual wavelengths are produced using different wavelength filters and a rotating reflective disk that direct the light to each of the respective filters. The reflective disk contains a notch that allows light to pass through it and two reflective surfaces that reflect light. As the disk rotates, the light is appropriately distributed to the respective filters. The rotation of the reflective disk is synchronized to a video camera that is used to image fluorescence. The camera is designed so that each of the wavelengths are giving alternating video frames.
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