| 摘要 |
A b s t r a c t A method for the specific multiplication and for the detection of nucleic acid templates by hybridization of two nucleic acid primers to this template, formation of a nucleic acid complementary to the template with these primers and transcription of the nucleic acid formed into a multitude of nucleic acids analogous to the template, characterised in that the template is hybridized with two primers which are arranged in the same orientation, which contain sequences complementary to the template and of which the second primer carries, at the end facing away from the first primer, a transcription-initiation site and a double-stranded sequence to which a RNA polymerase can bind, the two primers hybridized in this way are covalently linked to form a nucleic acid complementary to the template by filling up the gap between them, transcripts of this nucleic acid complementary to the template are formed and, if desired, these transcripts are detected in a known manner.
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