| 摘要 |
PURPOSE:To obtain the subject substance at an excellent production rate by culturing a levan-producing bacillus belonging to Serratia genus. CONSTITUTION:By separation from the soil, a levan-producing bacillus, Serratia levanicum [Serratia sp.NOC 1219 (FERMP-11171)] having the following mycological properties is obtained; Shape: rod bacillus having (0.6-0.8)X(1.0-2.0)mu; no mobility; growth temperature: 8-41 deg.C; growth pH: 4.0-11.5; H2S production: negative; gas production from glucose: negative, etc. The obtained bacillus is then inoculated into a culture medium containing a hydrocarbon source such as an alcohol, a nitrogen source such as urea, an inorganic salt, etc., such as an Mg salt and aerobically cultured under aeration-agitation, etc., at pH3.5-9.5, at 20-40 deg.C for 1-5 days to obtain a cultured material. The resultant cultured material is diluted using about double amount of water and centrifuged at about 10000G for about 10min. To the separated supernatant, isopropanol, etc., is added to obtain a precipitate. The resultant precipitate is then purified by dialytic method, etc., thus producing the objective levan. |
