发明名称 |
METHOD OF JUDGING TYPE OF HUMAN LEUKOCYTE ANTIGEN |
摘要 |
A method of judging the type of a human leukocyte antigen (HLA) which is a human histocompatible antigen playing an important role in tissue transplantation and diagnosis of autoimmune diseases and drug sensitivity, said method comprising hybridizing a probe labeled with an acridinium ester through a linker arm under hybridization conditions to the product of amplification of either cDNA prepared from mRNA contained in the specimen or genomic DNA contained in the specimen; decomposing selectively the labeled portion of the unhybridized probe and that of the probe which is hybridized but has mismatching of one or more bases in the crossing; and determining the residual chemiluminescence. This method makes it possible to judge the subtype of the class II gene of HLA, which has been serologically impossible heretofore. <IMAGE> |
申请公布号 |
AU7211191(A) |
申请公布日期 |
1991.09.18 |
申请号 |
AU19910072111 |
申请日期 |
1991.02.08 |
申请人 |
CHUGAI SEIYAKU KABUSHIKI KAISHA |
发明人 |
KOICHI MATSUBARA |
分类号 |
G01N33/53;C12Q1/04;C12Q1/68;C12R1/00;G01N33/80 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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