19F LABELLED COMPOUNDS AS NMR IMAGING AND SPECTROSCOPY AGENTS

摘要

Oxygen tension of tissue in a living subject may be determined non-invasively by a method which involves: administering to a living mammalian subject a biologically compatible perfluorocarbon emulsion in an amount effective to generate a measurable 19F spectrum under 19F NMR spectroscopy; allowing sufficient time to elapse for substantially all of the perfluorocarbon emulsion to be cleared from the vascular system of the subject, with a portion of the perfluorocarbon emulsion becoming sequestered in tissue of the subject; subjecting the tissue in which the perfluorocarbon emulsion has become sequestered to a 19F magnetic resonance spectroscopy procedure in which simultaneous measurements are made of spin-lattice relaxation rates for at least two separate resonances of the perfluorocarbon emulsion; and comparing the at least two spin-lattice relaxation rates measured in the 19F magnetic resonance spectroscopy procedure to a predetermined relation of spin-lattice relaxation rate to oxygen tension and temperature for the perfluorocarbon emulsion used, and thereby determining the oxygen tension and temperature of the tissue.