发明名称 |
METHOD OF REGENERATING RICE PLANT FROM PROTOPLASTS |
摘要 |
Method of generating a complete plant of rice from a protoplast thereof. To prepare the protoplast, a callus is derived from an embryo, scutellum, or radicle of a seed of rice. The callus is then cultured in a liquid medium to form a small cell cluster which readily releases a protoplast. The cell cluster is then treated with a protoplast-releasing enzymatic solution to obtain a protoplast. The protoplast is then cultured to form a callus, and the callus is then cultured in a differentiation medium to regenerate a whole plant of rice. |
申请公布号 |
CA1288713(C) |
申请公布日期 |
1991.09.10 |
申请号 |
CA19860509543 |
申请日期 |
1986.05.20 |
申请人 |
MITSUI TOATSU CHEMICALS INC. |
发明人 |
FUJIMURA, TATSUHITO;SAKURAI, MOTOI;AKAGI, HIROMORI;NEGISHI, TOMOKO;HIROSE, AKIRA |
分类号 |
C12N15/02;A01H4/00;C12N5/00;C12N5/02;C12N5/04;C12N5/10 |
主分类号 |
C12N15/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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