| 摘要 |
Recombinant haemoglobin is produced in yeast by co-expression of alpha -globin and beta -globin, the amount of alpha -globin mRNA and/or of alpha -globin protein produced exceeding that of beta -globin mRNA or beta -globin protein, respectively. The co-expression may be achieved by having a common upstream activation site (e.g. the GAL1/GAL10 UAS) regulating divergently arranged promoters for divergently transcribed alpha - and beta -globin coding sequences. The manipulation of the relative amounts of alpha and beta mRNA may be achieved by having the respective coding sequences on separate plasmids with differing copy numbers or by choosing respective promoters for the two coding sequences with differing strengths. |
