发明名称 |
TWO-DIMENSIONAL ELECTROPHORETIC SEPARATION OF CARBOHYDRATES |
摘要 |
Separation methodology is disclosed which allows for the separation of mixtures of carbohydrates into highly resolved detectable bands of carbohydrates. The method involves first reacting a mixture of carbohydrates with charge generating moieties which are capable of fluorescing such as 1-amino-4-naphthalene sulfonic acid (ANSA) to form carbohydrate conjugates. The conjugates are subjected to a first-dimensional gel electrophoresis in a first direction to provide separate bands of carbohydrates in the gel. A band in the gel is removed and subjected to second-dimensional electrophoresis in a second direction which is substantially perpendicular to the first direction. More specific bands of more highly resolved carbohydrates are then formed in the second-dimensional gel. The more specific bands within the second-dimensional gel are then electro-blotted onto a substrate surface and can be viewed in extremely small amounts due to the fluorescent capability of the ANSA when viewed under ultraviolet light. |
申请公布号 |
AU6901291(A) |
申请公布日期 |
1991.09.03 |
申请号 |
AU19910069012 |
申请日期 |
1990.11.21 |
申请人 |
GLYCOMED INCORPORATED |
发明人 |
BRIAN K. BRANDLEY;ROBERT J STACK |
分类号 |
C07H1/06;C13B20/18;G01N27/447 |
主分类号 |
C07H1/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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