发明名称 |
Factor VIII:C cDNA cloning and expression |
摘要 |
Methods and compositions are provided for recombinant DNA production of Factor VIIIC and truncated derivatives thereof. Based on amino acid sequences, probes are developed for isolating messenger RNA, cDNA and/or chromosomal DNA encoding for Factor VIIIC. The Factor VIIIC gene in its entirety, a fragment thereof, or a cDNA is then used for expression of Factor VIIIC in a host. The bacteriophage lambda FVIII23D containing the 14.43kb EcoRI fragment was deposited at the A.T.C.C. on Jan. 4, 1984 and given Accession No. 40094. Also, the vector pSVF8-200 was deposited at the A.T.C.C. on July 17, 1985 and given Accession No. 40190.
|
申请公布号 |
US5045455(A) |
申请公布日期 |
1991.09.03 |
申请号 |
US19900538691 |
申请日期 |
1990.06.14 |
申请人 |
CHIRON CORPORATION |
发明人 |
KUO, GEORGE;MASIARZ, FRANK;TRUETT, MARTHA;VALENZUELA, PABLO;RASMUSSEN, MIRELLA E.;FAVALORO, JENNIFER M.;CAPUT, DANIEL;BURKE, RAE L.;PACHL, CAROL |
分类号 |
A61K38/00;C07K14/755;C07K16/36;C12N1/21;C12N15/12;C12Q1/68 |
主分类号 |
A61K38/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|