<p>A method of human HLA-DR and/or Dw allotype matching comprises: (i) effecting polymerase chain reaction (PCR) amplification of a HLA-DRB gene exon 2 nucleotide sequence of a first sample of DNA; (ii) separating according to size the DNA fragments resulting from the said amplification; (iii) determining the length polymorphism of the thus separated DNA fragments; and (iv) comparing the thus-determined length polymorphism with the length polymorphism of DNA fragments resulting from PCR amplification of the said HLA-DRB gene exon 2 nucleotide sequence of a second sample of DNA. u</p>
申请公布号
EP0443748(A2)
申请公布日期
1991.08.28
申请号
EP19910300965
申请日期
1991.02.06
申请人
NATIONAL UNIVERSITY OF SINGAPORE
发明人
HUI, KAM MAN, C/O INSTITUTE OF MOLECULAR AND;BIDWELL, JEFFREY LEWIS