| 摘要 |
PURPOSE:To obtain a circulating culture device capable of effectively culturing a plant cell, tissue or fine algae, etc., by constituting the circulating culture device so as to carry out air stirring and light irradiation using a helical tube structure part provided in a part of circular culture tank for circulating culture liquid containing cells to be cultured. CONSTITUTION:In the above-mentioned helical tube structure part 2A, cells to be cultured in culture medium passing through the structure part 2A are irradiated with light from a light source 10 provided in the inner circumference part and/or outer circumference part and simultaneously the culture medium is stirred by blowing a gas such as O2 or CO2 from a gas blowing port provided in the lower part of the structure part 2A and, at the same time, foam is continuously raised in the inside of a tube. Thereby the cells to be cultured are stirred without precipitating the cells in the inside of the tube. Consequently, light can be uniformly and sufficiently irradiated to the cells. Moreover, physical stress of the cells suffered by the stirring is reduced and the cells can be efficiently reared and proliferated, and further, the secondary metabolites can be produced in high yields.
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