CALBOMYCIN-BIOSYNTHESIZED GENE CAR L AND CAR M FOR USE IN STREPTOMYCES GENUS AND OTHER MICROOGANISMS

摘要

The carL and carM genes of Streptomyces thermotolerams have been isolated and used to construct recombinant DNA expression vectors. The carL and carM encode activities required for the biosynthesis of macrolide antibiotics. These genes have been shown to complement Streptomyces fradiae mutants which lack the ability to produce the antibiotic, tylosin. The carL and carM genes can be used not only to construct recombinant cells with increased ability to produce macrolides but also to construct recombinant cells with the ability to produce novel antibiotic compounds.