发明名称 |
STRAIN RAL-4, USEFUL FOR PRODUCTION OF SMALL PROTEINS AND PEPTIDES |
摘要 |
This invention presents a novel strain of E. coli, RAL-4, which is a tryptophan auxotroph and shows enhanced expression of proteins and peptides. In addition, this invention presents a method for achieving enhanced expression of proteins and peptides either by the use of RAL-4 or via generation of a host cell auxotrophy for an amino acid not resident in the heterologous protein to be expressed. In an embodiment of the invention, IGF-I and GRF are exprressed at levels above that found in wild type controls |
申请公布号 |
WO9111523(A1) |
申请公布日期 |
1991.08.08 |
申请号 |
WO1991US00265 |
申请日期 |
1991.01.18 |
申请人 |
THE UPJOHN COMPANY |
发明人 |
LEPLEY, ROBERT, ALAN;OLSON, ERIC, ROBERT |
分类号 |
C12N1/21;A61K38/00;C07K14/60;C07K14/65;C12N15/09;C12N15/70;C12N15/71;C12P21/02;C12R1/19 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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