| 摘要 |
PURPOSE:To automatically perform enzyme immunoassay using a pH electrode by providing a cartridge wherein a dilution cup in which a pipette chip-shaped dilution fine diameter tube is received and a reaction cup are integrally arranged. CONSTITUTION:A specimen and a diluent are injected in a dilution cup 3 to prepare a specimen solution diluted in predetermined dilution magnification. Next, the leading end part of the fine diameter tube 8 for a solid phase received in a reaction cup 4 is immersed in the specimen solution in the dilution cup 3 to perform primary immunoreaction. Subsequently, a dissolving solution is injected in the reaction cup 4 to dissolve the freeze-dried enzyme labelled reagent of the reaction cup 4 and, thereafter, the leading end part of the fine diameter tube 8 after primary immunoreaction is immersed in the enzyme labelled reagent solution to perform secondary immunoreaction. Next, the fine diameter tube 8 after the secondary immunoreaction is washed. The washed fine diameter tube 8 is allowed to cover the responsive part of the pH electrode 65 provided in a measuring cell filled with a substrate solution and the pH change accompanied by the decomposition reaction of the substrate solution between the fine diameter tube 8 and the responsive part of the pH electrode 65 is measured. |
