| 摘要 |
PURPOSE:To prepare the titled substance in a short time, by cultivating cells of a plant belonging to the genus Cephalotaxus, producing and accumulating the titled substance in the culture, and collecting the titled substance. CONSTITUTION:A stem, leaf, root or other tissual slices or cellular groups of a plant belonging to the genus Cephalotaxus of the family Cephalotaxaceae, e.g. Cephalotaxus harringtonia or Cephalotaxus drupaceae Sieb. et Zucc. var. koraiana Makino, are sterilized, and then cultivated in the Murashige and Skoog culture medium to induce calluses. The resultant calluses are then cultivated on a solid culture medium or in a liquid culture medium by the same method as in the ordinary cultivation of microorganisms to multiply the aimed substance expressed by the formula. The aimed substance is separated by freeze-drying the cultivated cells, adding 95% ethanol thereto, and extracting the aimed substance at 30-35 deg.C. |
