PURIFICATION OF PHAGE DNA

摘要

PURPOSE:To enable to highly purify the DNA of a phage without requiring a centrifugal separation operation and an injurious reagent by removing the cells of mixed microorganisms, decomposing and denaturing phage protein and subsequently subjecting the phage to an ultrafiltration. CONSTITUTION:A mixture solution containing microorganisms and phages is filtered with a membrane filter to remove the microorganisms. The proteins of the remaining phages are decomposed and denatured and removed by an ultrafiltration method to purify the DNA of the phages. The pore size of the membrane filter is preferably approximately 0.45 to 0.22mum according to a relation between the permeation-inhibiting degree and filtration rate of the cells. A method for decomposing and denaturing the protein includes a method in which the protein is treated with a proteinase such as proteinase K.