| 摘要 |
A method for the amplification of at least a part of a target nucleic acid fragment/vectorette unit, said part comprising i) unknown sequence, ii) a first priming region of known nucleotide sequence for hybridisation with a first primer and iii) a vectorette portion at least one strand of which has a nucleic acid sequence which in either its double stranded complementary form or its single stranded form is capable of being bound by protein, which method comprises treating the target nucleic acid fragment/vectorette unit, together or sequentially, with appropriate nucleoside triphosphates and an agent for polymerisation of the nucleoside triphosphates under hybridising conditions, such that an extension product of a first priming region is synthesised complementary to a single stranded target nucleic acid/vectorette unit having a first priming region to which is hybridised a first primer selected so as to be substantially complementary to the first priming region, whereas no such extension product is synthesised complementary to single stranded target nucleic acid fragment/vectorette units having no such first priming region. |
