Process for the production of thrombin and high purity thrombin preparation thereby obtained.

摘要

A process for the production of a liquid thrombin preparation comprises reacting each unit of prothrombin with less than 50% of the conventional thromboplastin input in the presence of calcium, contacting the resultant thrombin with a phosphate buffer, and diluting and filtering the suspension. The filtrate is then applied sequentially to an anion-exchange agarose column and a cation-exchange agarose column and the thrombin fraction is step-wise eluted from the latter column with phosphate buffered saline. Liquid thrombin preparations thereby obtained have specific activities greater than 1,600 U/mg and can be used in haemostasis.