| 摘要 |
<p>PURPOSE:To produce and use acidic urethanase, capable of decomposing ure thane in an acidic range, readily or inexpensively by culturing a strain of spe cific bacterium. CONSTITUTION:A strain of bacterium such as Bacillus licheniformis asp.1013 (FERM P-1,1104) separated from contents in hat digestive tract is aerobically cultured in a medium containing a nitrogen source such as meat essence, a carbon source such as glucose and an inorganic salt such as potassium primary phosphate to produce urethanase. Then, since the produced urethanase exists in the cell, urethanase is extracted by a well-known method such as cell grinding method or extraction method and purified by chromatography. The prepared acidic urethanase has optimum pH at pH about 2.6-7.5 and has enzyme activity of decomposing urethane. Consequently, ethyl carbamate in fermented food industry (acidity) is made harmless by decomposition and acidic urethanase contributes to healthy and hygienic field of fermented foods.</p> |
