Method and kits for detecting human leukocyte antigen DNA.

摘要

A rapid and simple method for detecting HLA DNA comprises amplifying HLA DNA using polymerase chain reaction in which at least one primer is biotinylated, contacting denatured amplified DNA strands with an oligonucleotide probe, and detecting the insolubilized materials using a peroxidase-avidin conjugate. The probe comprises a polymeric particle and an oligonucleotide which is complementary to the amplified product, is 15 to 25 nucleotides in length, and is attached to the particle through a linking group having 16 to 30 ethylene glycol units. This method is used to advantage to type HLA, or to detect HLA DNA in a specimen. The materials needed to carry out the method can be provided in test kits. Desirably, the assay is carried out using a disposable test device having a microporous filtration membrane.