COLORIMETRIC DETERMINATION OF CHLORIDE IONS

摘要

The invention relates to a method for measuring chloride ion concentration in a sample of body fluid, for example, serum or plasma in which a sample blank is made in the same cuvette after the test measurement has been made; which method comprises the steps of: 1) Mixing a sample of body fluid with a water-soluble color reagent containing Fe+++, Hg++ and SCN- ions: 2) Measuring the absorbance of the mixture of Step 1 at from about 450 to about 550 nm: 3) Adding an iron complexing reagent to the mixture of Step Z wherein said iron complexing reagent complexes iron and. forms essentially colorless complexes with Fe+++ and Hg++; and 4) Measuring the absorbance of the mixture of Step 3 at from about 450 to about 550 nm. The di the first absorbance measurement is proprotional to the chloride ion concentration in the sample.