IMMUNOASSAY OF PROTEIN A UNDER ACIDIC CONDITIONS

摘要

The present invention features an improvement in a method for detecting protein A in an IgG-containing sample in a liquid medium, which method utilizes anti-protein A antibody. The improvement involves buffering the liquid medium, when the sample is contacted with the anti-protein A antibody for the purpose of promoting binding between the protein A in the sample and the protein A antibody, with a buffer to a pH at which protein A dissociates from the Fc region of the IgG.