GENETIC ASSAY FOR CYTOCHROME P450 IID BUFURALOL-1'

摘要

Polymorphisms at positions 100, 271, 281, 294, 408, 506 or 1432 of the cytochrome P450 enzyme bufuralol-1'-hydroxylase are indicative of the extensive metaboliser/poor metaboliser phenotypes and can be detected using known methods such as am- plification of the DNA with the polymerase chain reaction, followed by digestion with a suitable restriction enzyme and analysis by gel electrophoresis. The EM/PM phenotype is relevant to calculating safe or effective drug doses for individuals.