TUMOR-SPECIFIC, CELL SURFACE-BINDING MONOCLONAL ANTIBODIES

摘要

PCT No. PCT/US90/07496 Sec. 371 Date Aug. 21, 1992 Sec. 102(e) Date Aug. 21, 1992 PCT Filed Dec. 18, 1990 PCT Pub. No. WO91/09135 PCT Pub. Date Jun. 27, 1991.Disclosed is a process for the preparation and use of gynecological tumor diagnostic and antitumor reagents. The process involves the pre-treatment of a patient with a viral oncolysate and the establishment of stable B cell human hybridomas capable of producing human monoclonal antibodies reactive with cell surface epitopes of human gynecological tumors. At least one such surface epitope is described as is the association constant of the antibody for certain gynecological tumor cells. Also disclosed are methods for utilizing the monoclonal antibodies of the invention in diagnoses and treatment of gynecological malignancies. In addition, two particularly useful gynecological hybridoma lines are disclosed which were derived from the process of the invention.