摘要 |
An improved continous countercurrent chromatography apparatus comprising a separation column provided with first and second ends, feed conduit means for introducing fluids to the first end of the separation column, fluid monitoring means (1) provided with first and second ends, and delivery conduit means connecting the second end of the separation column to the first end of the fluid monitoring means (1) having a light source (2), a flow cell compartment (4), and a detector (5), the improvement comprising a thermal regulator means positioned between the delivery conduit means and the monitoring means (1), where the regulator means is capable of maintaining a pre-determined temperature, and means for applying back-pressure to the fluid exiting the monitoring means (1), where the back-pressure means is connected to the second end of the monitoring means (1). |