发明名称 |
Process for producing 7-aminocephem compound or salts thereof. |
摘要 |
A process for producing 7-aminocephem compounds, or salts thereof, which comprises cultivating in a nutrient medium a microorganism capable of producing a 7-aminocephem compound of the formula: <CHEM> wherein R is acetoxy, hydroxy or hydrogen, and recovering the compound (I) or a salt thereof from the culture broth. An example of a suitable 7-aminocephem compound-producing microorganism is a fungal strain belonging to the species Acremonium chrysogenum that is capable of producing a cephalosporin compound of the formula: <CHEM> wherein R is as above and X is -CH(NH2)-COOH, -CO-COOH or -COOH, said fungal strain having been transformed with a 7-aminocephem compound production vector containing one or more promoters for Acremonium chrysogenum and gene(s) connected therewith which encodes enzyme(s) capable of converting the cephalosporin compound (II) to the 7-aminocephem compound (I). A vector for use in the process, cephalosporin compound-producing microorganisms transformed with said vector and a DNA fragment having the promoter activity of the Acremonium chrysogenum alkaline protease gene are also described. |
申请公布号 |
EP0436355(A2) |
申请公布日期 |
1991.07.10 |
申请号 |
EP19900313988 |
申请日期 |
1990.12.20 |
申请人 |
FUJISAWA PHARMACEUTICAL CO., LTD. |
发明人 |
ISOGAI, TAKAO;FUKAGAWA, MASAO;IWAMI, MORITA;ARAMORI, ICHIRO;KOJO, HITOSHI |
分类号 |
C12N15/09;C12N1/15;C12N1/21;C12N9/06;C12N9/58;C12N9/80;C12N15/55;C12N15/57;C12N15/80;C12P17/00;C12P17/18;C12P35/02;C12R1/01;C12R1/645 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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