CLONING VECTOR FOR USE IN LACTIC ACID BACTERIA

摘要

A food-grade innocuous cloning vector for use in lactic acid bacteria comprises a replication region with a narrow host range, preferably the replication region of a 28 kbp plasmid from Lactococcus lactis subsp. lactis biovar. diacetylactis SSD207, and a bacteriocin resistance determinant, preferably the nisin resistance determinant from plasmid pSW211 obtained from Lactococcus lactis subsp. lactis 10.084, as a selectable marker. With this vector it is possible to construct genetically engineered products (i.e. organisms or chemical products) which are safe enough to be a part of human food. Neither those bacteria in which the vector can replicate, nor the DNA which makes up the vector offer any hazard to human health.