RECOMBINANT EXPRESSION VECTORS AND PURIFICATION METHODS FOR THERMUS THERMOPHILUS DNA POLYMERASE

摘要

Recombinant DNA sequences encoding the DNA polymerase I activity of Thermus thermophilus can be used to construct recombinant vectors and transformed host cells for production of the activit y, T. thermophilus DNA polymerase I is an ~ 94 kDa protein especially useful in the DNA amplification procedure known as the polymerase chain reaction.