METHODS FOR PURIFICATION OF SINGLE-CHAIN AND DOUBLE- CHAIN TISSUE PLASMINOGEN ACTIVATOR

摘要

A mixture containing a single-chain tissue plasminogen activator (tPA) and/or double-chain tPA is brought into close contact with a column carrying an immobilized Erythrina trypsin inhibitor as an affinity agent. Adsorbed proteins are eluted with eluents having different pHs with or without arginine or benzamidine, so that single-chain tPA is obtained in the eluent with a pH at least 4.5 and double-chain tPA is obtained in the eluent with a pH lower than 4.5.