摘要 |
PURPOSE:To obtain the title cullulase higher in crystalline cellulose decomposing activity and filter paper decomposing activity in comparison with CMC decomposing activity by culturing a specific Bacillus circulans in a culture medium and producing and accumulating cellulase in the culture medium. CONSTITUTION:Bacillus circulans LP-547 strain (FERM P-10970) is cultured in a culture medium and a cellulase having higher crystalline cellulose composing activity and filter paper decomposing activity in comparison with CMC decomposing activity is produced and accumulated and then collected. Furthermore, the culture is preferably e.g. carried out at about pH7.0-9.0 and 30-37 deg.C for 24-90hr. |