摘要 |
Isopenicillin N epimerase is provided in purified form following isolation from cell-free extracts of Streptomyces clavuligerus. The enzyme of molecular weight of 47,000 (SDS-PAGE) and 50,000 (gel filtration) has been purified to electrophoretic homogeneity, is monomeric, and contains 1 mole of pyridoxal-5 min -phosphate per mole of protein. |