| 摘要 |
PURPOSE: To achieve a rapid analysis by analyzing a substrate surface with polynucleotide with a resolution equal to or less than an atom diameter, reproducing the type of each continuous base of polynucleotide from collected data, and identifying and recording the type. CONSTITUTION: With a microscope, its end, part forms a tip that is approximately a single atom, and the move includes a probe 2 that is constituted of a needle being assured by piezoelectric crystals Px-Pz. The crystals Px and Py are operated by scanning devices 3 and 4 in X and Y directions, and the crystal Pz is operated so that it constantly maintains current that flows between the probe 2 and polynucleotide on a substrate by a servo controller 5. Then, coordinates X-Z at the edge part of the probe 2 are sampled by a sampler 10 and are inputted to a processing device 11. Then, in the device 11, the image of a substrate atom and atoms of, for example, phosphoryl are eliminated from the obtained image, purine nucleus or pyrimidine nucleus and two types of purine type bases are mutually distinguished on the image, each base is continuously identified, and the obtained result is stored in a recording device 15. |
