| 摘要 |
The present invention relates to a method in which the polymerase chain reaction (PCR) is used to generate products that form double-stranded DNA with discrete, cohesive, single-stranded ends when said products are combined, denatured, and reannealed. Such cohesive ends can be used to form DNA joints, as well as circular DNA. Furthermore, the above method can be utilized in site-specific mutagenesis, in DNA recombination, and in other additional techniques. |
