Immunoaffinity column for simultaneous extraction of several anabolic hormone residues

摘要

A column for the simultaneous extraction of several anabolic hormone residues from biological liquids or biological tissue homogenates digested with proteolytic enzymes contains two or more gel fractions having a single polysaccharide matrix but each conjugated with a specific immunoglobulin for one of the hormones to be isolated. Conjugation may be effected by incubating an aqueous solution of said immunoglobulin with an aqueous suspension of a respective gel fraction for a time of between 3 and 12 hours at a temperature of between 4 and 25 DEG C.