| 摘要 |
The present invention is directed to bacteria that overproduce riboflavin. The invention concerns the nucleotide sequence of the rib operon and its open reading frames, and recombinant bacteria that contain the rib operon. Specifically, the invention relates to bacteria that have been mutated so that their production of riboflavin and/or purines is deregulated, and which have copies of the rib operon inserted and amplified in their chromosomal DNA. In a specific embodiment, the rib operon itself can be deregulated by replacing its control regions with sequences that allow constitutive or unregulated expression. The bacteria, operons and sequences of this invention are used to overproduce relatively large amounts of riboflavin by fermentation. |
