PROCESS FOR PREPARING GANGLIOSIDE GM1

摘要

The invention relates to a process for preparing ganglioside GM1 by the action of neuraminidase isozyme S on a ganglioside. The neuraminidase isozyme S used is one obtained from a culture medium of bacterial of the genus Arthrobacter and having the following physicochemical properties; function: capable of selectively producing ganglioside GM1 from gangliosides; molecular weight: about 52000 daltons (according to gel filtration chromatography and SDS-PAGE electrophoresis); optimum pH: 3.8 to 4.4 (in the case of using bovine cerebroganglioside as the substrate); and thermal stability: up to 60 DEG C.