发明名称 |
RECOMBINANT DNA METHOD AND VECTORS FOR USE THEREIN |
摘要 |
There is described a method, for obtaining a eukaryotic cell containing in its DNA multiple copies of a GS gene, comprising: transforming a eukaryotic glutamine auxotroph with a GS gene; selecting transformant cells containing the GS gene; and culturing the selected transformant cells in a medium which lacks glutamine or in which the amount of glutamine is progressively depleted, the GS gene being of a character such that, or the conditions employed during the culturing step being such that, the GS gene is so weakly transcribed that cells in which the GS gene has been amplified are selected. |
申请公布号 |
WO9106657(A1) |
申请公布日期 |
1991.05.16 |
申请号 |
WO1990GB01640 |
申请日期 |
1990.10.25 |
申请人 |
CELLTECH LIMITED |
发明人 |
BEBBINGTON, CHRISTOPHER, ROBERT;YARRANTON, GEOFFREY, THOMAS |
分类号 |
C12N5/10;C12N15/09;C12N15/85 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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