INHIBITOR OF LAMININ NEURITE PROMOTING ACTIVITY

摘要

The invention provides a purified proteoglycan-associated inhibitor of the neurite-promoting activity of laminin and a purified compound which: a) inhibits the neurite-promoting activity of purified rat, mouse, and human laminin; b) is active whether presented to laminin in solution or after either the inhibitor or laminin is first bound to the culture substratum; c) does not act by displacing laminin from the substratum; d) can be prevented from binding to neurite-promoting laminin substrates by polyclonal and some monoclonal anti-laminin or polyclonal anti-entactin antibodies if the laminin contains associated entactin; e) can be purified from rat RN22 Schwannoma cells; and f) neurite-promoting activity can be abolished by proteases or glycosaminoglycan lyases but not by heat at 90 DEG for 15 minutes.