| 摘要 |
The invention is a method for reproducing coniferous trees by somatic embryogenesis using plant tissue culture. It comprises a multistage process in which a fertilized immature-embryo is excised and cultured to induce early state proembryos, which may be further cultured in liquid media without auxins or cytokinins but with the addition of abscisic acid, to develop late stage proembryos under significantly higher osmotic conditions of about 450 mM/kg. The medium may have added exogenous abscisic acid and must contain an absorbent material. After several weeks in culture the vigor and morphology of the embryos developed by the process are improved and the tendency of premature germination is reduced. The somatic embryos may be germinated before or after storage and transplanted to soil for further growth. |
